This material was loaded onto a silica gel column and the column was eluted with chloroform several times until the fraction of interest was collected . finally we obtained some light yellow green ointment liquid f i 采用硅膠作干層析柱填充物,用氯仿作展開劑和洗脫液,反復硅膠層析,對柄海鞘鞘囊氯仿浸提物進行分離、提取與純化,最后得到一淺黃綠色的油狀液體fraction (以下均稱f ) 。